The nitric oxide pathway requires
phosphorylation of eNOS to liberate NO. We correlated mRNA/protein levels of
VEGF164, and phosphorylated Serine1179 of eNOS during ROP vasoproliferation.
Animals were exposed to oxygen (50/10% every 24-hours) for 14 post-natal (PN)days. Pups were continuously exposed to 20.9% ambient oxygen from PN15 and
enucleated on PN days 15, 18, and 20. ADPase stained retinas were analyzed for
degree of a vascularity/total retina area. Western-blotting quantified the
phosphorylation of eNOS at Ser1179, the ratio of total eNOS to ERK2. Immuno reactivity
of VEGF and of eNOS serine1177 determined tissue protein expression.
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